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This research was performed to investigate the developmental ability of mouse oocytes following cold-culture(4^{circ}C) in vitro. When the oocytes were fertilized after 10 hour cold-culture in D-PBS or Ham’s F10 with 0.3% BSA, the cleavage rage of the oocytes was not different in the rate of oocytes fertilized in vitro without cold-culture(126/189, 66.2% vs 88/133, 66.7%) and also the rate of embryos developed to blastocyst did not(123/189, 65.1% vs 82/133). But, when the time of the cold-cultre was extended from 10 to 24 hours, the rate of embryos developed to blastocyst was slightly decreased(73.5% vs 52.2%). However, when the oocytes were cultured for 10 and 24hours at 37^{circ}C, the rate of oocytes developed to blastocyst was significantly decreased than that of oocytes following cold-culture. By the results of this study, it’ll be possible to utilize effectively the cold-culture of the oocytes when in vitro fertilization is delayed.
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